Commensal Clostridia in the preterm gut as reservoirs of antimicrobial resistance: susceptibility profiles, and resistance genes.

ABSTRACT: The gut microbiome of preterm infants is highly vulnerable to perturbations. Members of the class Clostridia are among the first anaerobes colonizing the preterm gut, yet their ecological roles and antimicrobial resistance (AMR) properties remain poorly understood. We characterized 98 Clostridia isolates from fecal samples of preterm infants, spanning 17 species and 11 genera. Isolates were identified by MALDI-TOF and 16S rRNA sequencing, colonization levels were quantified, and antimicrobial susceptibility was assessed by disk diffusion and E-test. Resistance determinants were screened by PCR and sequenced. We focused on Clostridia that were present at low colonization levels (mean 5.3 log10 CFU g-1 of feces). While most isolates were susceptible to amoxicillin-clavulanic acid, imipenem,

SUBMITTER: Delannoy J 

PROVIDER: S-EPMC12728818 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature