In vitro culture of cumulus oocyte-complexes from early antral follicles in prepubertal and adult ewes.

ABSTRACT:

Abstract

The long-term in vitro culture (LIVC) of cumulus-oocyte complexes (COCs) derived from early antral follicles (EAFs) has shown significant potential in producing meiotically competent oocytes in adult ewes. However, the effectiveness of this approach in prepubertal ewes remains unexplored. This study aimed to evaluate the efficiency of the LIVC system in prepubertal ewes, addressing the challenge of limited EAF availability in adults. Both adult and prepubertal ewe ovaries were used to isolate COCs from EAFs (350-450 µm), which were then cultured in TCM199 supplemented with 0.15 μg/mL Zn++, 10-4 IU/mL FSH, 10 ng/mL estradiol, 50 ng/mL testosterone, 50 ng/mL progesterone, and 5 μM cilostamide. After five days of LIVC, a group of COCs underwent in vitro maturation, while othe

SUBMITTER: Ebrahimi M 

PROVIDER: S-EPMC12728946 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature