Study of FOXO1/pFOXO1, lncRNA ADAMTS9-AS2, and miR-96-5p in laryngeal squamous cell carcinoma.

ABSTRACT:

Background

Laryngeal squamous cell carcinoma (LSCC) is recognized as the second most common malignant tumor of the respiratory tract. The study aimed to identify the roles of FOXO1, hsa-miR-96-5p, and lncRNA ADAMTS9-AS2 in the molecular pathogenesis of LSCC patients based on the systems biology data.

Methods

The LSCC patient tissue samples (n = 50) and the same individual's adjacent normal tissues (n = 50) were collected from the candidates (aged 57.75 ± 9.3 years) of surgery. The miR-96-5p and lncRNA ADAMTS9-AS2 were predicted using the specific servers. The Kaplan Meier analysis was employed using TCGA data. The FOXO1and ncRNA gene expression levels were measured with the RT-qPCR technique. The Western blot technique was applied to estimate FOXO1/pFOXO1 protein values.

SUBMITTER: Bakhshandeh M 

PROVIDER: S-EPMC12729057 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature