Ginsenoside Rh2 inhibits mesenchymal stem cell senescence by regulating mitochondrial and lysosomal function.

ABSTRACT:

Background

Mesenchymal stem cells (MSCs) undergo senescence after expansion and in vitro culture under oxidative stress, which limits their clinical application. Ginsenoside Rh2 has been confirmed to regulate mitochondrial function, but its role in modulating the senescence of MSCs has not been clearly investigated.

Purpose

This study aims to explore the effects and underlying mechanisms of Rh2 in inhibiting the senescence of MSCs.

Methods

Transmission electron microscopey (TEM) and fluorescence staining assays were used to monitor changes in mitochondrial and lysosomal morphology and function in Rh2-treated senescent MSCs. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot analyses were performed to evaluate the expression levels

SUBMITTER: Zhuang J 

PROVIDER: S-EPMC12729120 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature