Immunogenicity of a bivalent recombinant adenovirus expressing VP1 proteins of bovine norovirus and bovine nebovirus in mice and calves.

ABSTRACT: Bovine norovirus (BNoV) and bovine nebovirus (BNeV) are emerging pathogens responsible for severe diarrhea in calves worldwide. In this study, we constructed a bivalent recombinant adenovirus vector vaccine, designated rAd5-BNoV + BNeV_VP1 encoding the genes for BNoV-VP1 and BNeV-VP1 based on replication-deficient human adenovirus type 5 (rAd5), and evaluated its immunogenicity in mice and calves. Serum antibody responses were assessed by enzyme-linked immunosorbent assay (ELISA) and serum blocking titer 50 (BT50). Results demonstrated that mice immunized with rAd5-BNoV + BNeV_VP1 produced significant VP1-specific IgG titers as early as two weeks post-vaccination. BNoV-VP1-specific IgG antibody titers peaked at 1:10⁵ in both intramuscular (I.M.) and oral groups (n = 6), while BN

SUBMITTER: Chen T 

PROVIDER: S-EPMC12729173 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature