Deletion of sRNA0024 Reduces Virulence of <i>Pseudomonas plecoglossicida</i> and Alleviates Host Immune Injury in <i>Epinephelus coioides</i>.

ABSTRACT: Visceral white spot disease caused by Pseudomonas plecoglossicida severely threatens marine aquaculture, highlighting the need for effective control strategies. To clarify the role of a novel small RNA, sRNA0024, in bacterial pathogenicity, we constructed an sRNA0024 deletion mutant (ΔsRNA0024) and compared its phenotype and virulence with those of the wild-type strain NZBD9. In vitro assays showed that deletion of sRNA0024 did not affect bacterial growth but significantly reduced biofilm formation and adhesion. In vivo infection experiments in orange-spotted grouper (Epinephelus coioides) demonstrated that the ΔsRNA0024 mutant had a 3.8-fold higher 50% lethal dose (LD₅₀), improved host survival, and milder splenic lesions than the wild type. Histopathology and hos

SUBMITTER: Zhao L 

PROVIDER: S-EPMC12729329 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature