A Novel <i>Rhodotorula evergladensis</i> CXCN-6 Rich in Torularhodin and PUFAs with Potent Antioxidant and Anti-Inflammatory Activities.

ABSTRACT: Carotenoids and microbial lipids are valuable bioactive compounds with potent antioxidant and anti-inflammatory properties. Microbial biosynthesis provides a sustainable alternative to conventional plant extraction and chemical synthesis. Here, we report the isolation and characterization of a novel oleaginous yeast, Rhodotorula evergladensis CXCN-6, from the surface of Nymphaea 'Gorgeous Purple'. The strain displayed intense reddish-orange pigmentation due to intracellular carotenoid accumulation. HPLC and LC-MS analyses identified torularhodin as the predominant carotenoid (m/z 563.4 [M]⁺), while lipids were rich in linoleic (C18:2), oleic (C18:1), and α-linolenic (C18:3) acids. Under optimized fermentation, CXCN-6 yielded 63.56 mg/L torularhodin and 9.83

SUBMITTER: Park C 

PROVIDER: S-EPMC12729354 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature