SRRM4 Knockout Helps the Human Mesenchymal Stem Cell Line to Penetrate Decellularized Cancellous Bone.

ABSTRACT:

Background

Analyzing the human bone marrow microenvironment requires an in vivo model that reflects the human bone marrow microenvironment. Introducing a human bone marrow mesenchymal stem cell (MSC) line into decellularized cancellous bone (DCB) is a first step in forming such a bone marrow model. Our goal with this research is identifying factors that promote the penetration of MSCs into DCBs in an ex vivo setting.

Methods

We introduced the CRISPR Knock Out (GeCKO v2) library to identify candidate genes in UE7T-9 cell line (MSC line) for DCB penetration. We established a candidate gene-knockout UE7T-9 cell for validation and evaluated its penetration into DCB (measured distance of randomly selected 100 cells), proliferation (MTS assay), migration (scratch assay), and ancor

SUBMITTER: Onishi I 

PROVIDER: S-EPMC12729399 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature