Microencapsulation of β-Glucosidase in Alginate Beads for Post-Rumen Release in Ruminant Gut.

ABSTRACT: This study aimed to develop a microencapsulation formulation for efficient encapsulation of β-glucosidase to improve its stability in a rumen-like environment and sustain activity post-rumen in the ruminant gut. Various alginate-based formulations were evaluated to achieve high encapsulation efficiency (EE) and stability. These included control alginate beads (AB), microcapsules with chitosan (MCS), alginate-sucrose beads (AOS), alginate-sucrose-maltodextrin beads (AOMS), and alginate pectin beads (APB). The microcapsules were made using Buchi encapsulator B-390 with calcium chloride as the gelling solution. Alginate proved to be a suitable polymer for β-glucosidase encapsulation and <1 mm diameter microbeads were obtained across all formulations. Alginate alone (AB: 1% alginate, 0.2 U/mL

SUBMITTER: Almassri N 

PROVIDER: S-EPMC12729419 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature