Iron Chelation Reduces Intracellular Hydroxyl Radicals in Normal Human Dermal Fibroblasts Independently of Aging.

ABSTRACT: In cultured skin cells, decreases in antioxidant function and increases in intracellular free Fe²⁺ due to replicative aging have been reported. The Fenton reaction between Fe²⁺ and hydrogen peroxide is a threat to the skin because it produces hydroxyl radicals that attack proteins, nucleic acids and lipids. The purpose of this study was to determine whether exogenous iron modulation alters intracellular hydroxyl radicals in senescent normal human dermal fibroblasts (NHDFs). As previously reported, reduced antioxidant function, the accumulation of Fe²⁺ and increased levels of Reactive Oxygen Species (ROS) were observed in senescent NHDFs. The novel catalase (CAT) activity assay demonstrated a decrease in CAT activity alone in aged NHDFs. However, sufficient

SUBMITTER: Takemoto K 

PROVIDER: S-EPMC12729569 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature