3D Breast Cancer Spheroids Reveal Architecture-Dependent HER2 Expression and Signaling.

ABSTRACT:

Background

Three-dimensional (3D) culture systems offer a physiologically relevant alternative to monolayers for studying tumor organization, signaling, and drug response. HER2-positive breast cancers (BCa) account for 15-30% of BCa cases and benefit from HER2-targeted therapies, yet predictive in vitro models remain limited.

Objective

To generate and compare 3D spheroids from two HER2+ BCa cell lines, SKBR3 and BT474, and investigate how 3D architecture influences HER2 distribution, intracellular signaling, and cellular organization.

Methods

Spheroids were reproducibly generated from SKBR3 and BT474 cells and analyzed after 4 days of culture. Cell viability was evaluated using live/dead staining, HER2 distribution was assessed by confocal microscopy and quantified on

SUBMITTER: Arnaldi P 

PROVIDER: S-EPMC12729668 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature