The <i>SCD5</i> Gene Modulates Adipogenic Differentiation via the <i>WNT5B</i> Signaling Pathway in Xinjiang Brown Cattle.

ABSTRACT: This study investigated the role of SCD5 in bovine preadipocyte proliferation and adipogenic differentiation. SCD5 overexpression suppressed proliferation, reducing the percentage of EdU-positive cells and overall cell viability (p < 0.05). It also downregulated proliferative factors CDK1 and CDK2 (p < 0.05), and reduced lipid accumulation (p < 0.001) along with key adipogenic markers PPARγ, C/EBPα, and FABP4 (p < 0.01). Conversely, SCD5 knockdown promoted these processes. Mechanistically, SCD5 overexpression downregulated WNT5B (p < 0.05), while knockdown had the opposite effect. Silencing WNT5B significantly decreased β-catenin transcription (p < 0.05), total prot

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12729747 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature