Transcriptomic profiling of rumen epithelium, liver, and muscle reveals tissue-specific gene expression patterns in Hu sheep.

ABSTRACT:

Background

The rumen epithelium, liver, and muscle tissues are key metabolic organs in ruminants, each performing distinct yet interconnected roles in energy metabolism and growth. However, how these tissues coordinate their gene expression to meet metabolic demands in Hu sheep remains poorly understood.

Results

RNA sequencing of 48 tissue samples (rumen epithelium, liver, and muscle tissues from 16 male Hu sheep) identified 30,171 transcripts, including 7,403 commonly expressed and 3,414 uniquely expressed genes. The rumen epithelium displayed the highest number of uniquely expressed genes but lower functional enrichment compared to the liver and muscle, emphasizing its specialized yet limited metabolic pathways. Functional enrichment analysis showed that the rumen epitheli

SUBMITTER: Jia X 

PROVIDER: S-EPMC12729863 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature