Linoleic Hydroperoxides Are Potent Hyperoxidative Agents of Sensitive and Robust Typical 2-Cys Peroxiredoxins.

ABSTRACT: Typical 2-Cys peroxiredoxins (2-Cys Prxs, AhpC/Prx1 subfamily) are ubiquitous thiol peroxidases that efficiently reduce H₂O₂ and other hydroperoxides via a reactive peroxidatic Cys (C_P). Under elevated hydroperoxide levels, C_P can be hyperoxidized to sulfinic (C_P-SO₂H) or sulfonic (C_P-SO₃H) acids, leading to enzyme inactivation. Notably, eukaryotic 2-Cys Prxs are orders of magnitude more sensitive to hyperoxidation (sensitive Prxs) by H₂O₂ than their bacterial counterparts (robust Prxs). Sensitivity to hyperoxidation also correlates with the catalytic triad composition: enzymes containing threonine (Thr-Prx) are more prone to hyperoxidation by H₂O₂ than those

SUBMITTER: Cabrera VIM 

PROVIDER: S-EPMC12729873 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature