CircRNA_01754 Regulates Milk Fat Production Through the Hippo Signaling Pathway.

ABSTRACT:

Background

Milk fat metabolism is a complex process regulated by non-coding RNAs. circRNAs (circRNAs) can act as miRNA sponges to regulate target gene expression.

Purpose

This study aimed to investigate the role and mechanism of the circRNA_01754/miR-302c/LATS2 axis in regulating milk fat metabolism in bovine mammary epithelial cells (BMECs).

Methods

mRNA sequencing was performed on bovine mammary gland tissues from different lactation stages to analyze the expression profiles. The interactions between circRNA_01754, miR-302c, and LATS2 were verified using dual-luciferase reporter assays, qRT-PCR, and Western blot. The functional effects on triglyceride (TAG) and cholesterol synthesis were assessed.

Results

CircRNA_01754 was identified as a direct sponge for mi

SUBMITTER: Li X 

PROVIDER: S-EPMC12729982 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature