The Interaction Between PGD2 and G6PD6 Is Involved in Aromatic Amino Acid Synthesis.

ABSTRACT: The biosynthesis of AAAs in plants primarily relies on the shikimate pathway, with metabolic flux sustained by NADPH and E4P generated via the OPP pathway. However, how OPP enzymes coordinate to support AAA production remains unclear. Here, we investigated the direct interaction between two consecutive NADPH-producing enzymes, G6PD6 and PGD2, and its role in metabolic coupling. Using BiFC, Co-IP, pull-down assays, and domain mapping, we showed that G6PD6 and PGD2 form a cytosolic protein complex via the C-terminal domain of PGD2. Structural modeling identified potential interaction residues: PHE²⁹⁴, GLY²⁹⁷, and LEU²⁹⁸ in PGD2, and GLY³⁵¹, LYS⁴⁹⁹, and ALA⁵⁰⁰ in G6PD6. Overexpression of either enzyme partially rescued the dwar

SUBMITTER: Tang Q 

PROVIDER: S-EPMC12730105 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature