Comparison of digital PCR and real time PCR methods for quantitative analysis of African Swine Fever Virus.

ABSTRACT:

Introduction

The African swine fever (ASF) is a viral disease of significant socio-economic and animal health impact, commonly diagnosed using molecular techniques. The World Organization of Animal Health (WOAH) has recommended two highly sensitive and specific real time polymerase chain reaction (PCR) procedures (P1 qPCR and P2 qPCR) for the reliable and rapid detection of the ASF virus (ASFV). The present work aimed to standardize two third-generation PCR technologies by using the same WOAH-recommended primer/probe sets.

Methods

We developed two droplet digital PCR protocols ddPCRs and compared their analytical performances to the above-mentioned qPCRs. This involved testing serially diluted plasmid containing the vp72 gene sequence of the ASFV as the template. Clinical po

SUBMITTER: Dei Giudici S 

PROVIDER: S-EPMC12730159 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature