Definition and Discovery of Tandem SH3-Binding Motifs Interacting with Members of the p47^phox-Related Protein Family.

ABSTRACT: SH3 domains are widespread protein modules that mostly bind to proline-rich short linear motifs (SLiMs). Most known SH3 domain-motif interactions and canonical or non-canonical recognition specificities are described for individual SH3 domains. Although cooperation and coordinated motif binding between tandem SH3 domains has already been described for members of the p47^phox-related protein family, individual cases have never been collected and analyzed collectively, which precluded the definition of the binding preferences and targeted discovery of further instances. Here, we apply an integrative approach that includes data collection, curation, bioinformatics analyses and state-of-the-art structure prediction methods to fill these gaps. A search of the human proteome with the s

SUBMITTER: Kalman ZE 

PROVIDER: S-EPMC12730246 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature