Investigating the bioorthogonality of isocyanides.

ABSTRACT: Isocyanides have been applied in bioorthogonal reactions as triggers for uncaging reactions. However, emerging evidence suggests that they may label proteins covalently. We synthesised a fluorophore-conjugated isocyanide and analysed its protein reactivity. Our results suggest that isocyanides cannot be considered to be bioorthogonal in chemical biology.

SUBMITTER: Nakao R 

PROVIDER: S-EPMC12730495 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature