Rapid Visual Detection of Mycoplasma Hominis Using an RPA-CRISPR/Cas12a Assay.

ABSTRACT: Mycoplasma hominis (MH) is a prevalent opportunistic pathogen that is strongly associated with a wide range of urogenital tract infections and severe adverse pregnancy outcomes in clinical settings. Current MH detection methods, including microbial culture and qPCR, are time-consuming and rely on complex equipment, making them unsuitable for scenarios requiring rapid or simplified testing. In this study, we developed a visual readout biosensing platform by synergistically integrating recombinase polymerase amplification (RPA), CRISPR/Cas12a-mediated target nucleic acid recognition, and lateral flow biosensors for the rapid, sensitive, and specific identification of MH. The assay specifically targets the MH-specific 16S rRNA gene, achieving a limit of detection as low as 2 copies/reaction o

SUBMITTER: Chen J 

PROVIDER: S-EPMC12730632 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature