Development of Metabolite-Responsive Transcription Factor Systems as Modular Platforms for Gene Expression Control.

ABSTRACT: Traditional inducible systems typically induce the simultaneous expression of all genes controlled by similar promoters, thereby limiting their use. In this study, we used two metabolite-inducible systems, MarR from the Escherichia coli mar operon and TtgR from the Pseudomonas putida ttg operon, to assess their use as gene regulation platforms beyond reporter assays. Ligand-dependent transcription was validated using eGFP. The reporter was replaced with two flavonoid O-methyltransferases (OMTs), ROMT-9 and SOMT-2, under transcription factor (TF)-specific promoters. In E. coli, both systems enabled in using HPLC. TF-based expression did not impact enzyme activity. Induction with salicylic acid (MarR) produced stronger gains than that with 4'-hydroxyflavanone (TtgR), alt

SUBMITTER: Ji H 

PROVIDER: S-EPMC12730684 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature