Drug-Induced Partial Immunosuppression for Preclinical Human Tumor Xenograft Models.

ABSTRACT:

Background

With the rising incidence of cancer, there is a growing need for improved preclinical models to test new therapies. While patient-derived xenografts (PDX) in immunodeficient mice are the gold standard, they are costly and result in a complete absence of a functional immune system, limiting their utility for studying tumor-immune interactions. This study characterizes a pharmacological partial immunosuppression protocol in immunocompetent mice as a promising alternative, evaluating its impact on the immune system and demonstrating its efficacy for growing human tumor xenografts.

Methods

Mice received a regimen of cyclosporine (20 mg/kg, i.p., every 48 h for 12 days), cyclophosphamide (60 mg/kg, i.p., every 48 h for 8 days), and ketoconazole (10 mg/kg, p.o., for 12

SUBMITTER: Gorbushin AK 

PROVIDER: S-EPMC12730912 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature