Chromosome-Level Genome Assembly of Red Sea Bream (<i>Pagrus major</i>) Reveals Integration of Heterospecific Sperm-Derived Genetic Material in Artificial Gynogenesis.

ABSTRACT: Artificially induced gynogenesis, a technique that utilizes UV-irradiated sperm to activate eggs while excluding paternal genetic contribution, has been instrumental in the genetic improvement of aquaculture species. Although the allo-sperm effect has been observed in some freshwater fish and suggests the integration of paternal DNA, its occurrence and mechanisms in marine fish remain unclear. In this study, a 795.23 Mb chromosome-level genome assembly for red sea bream (Pagrus major) was presented, with a scaffold N50 of 32.03 Mb, encompassing 29,083 protein-coding genes. Furthermore, the allo-sperm effect was investigated on the artificial gynogenesis of Japanese flounder (Paralichthys olivaceus) induced by UV-irradiated P. major sperm. Whole-genome sequencing of gyn

SUBMITTER: Liu M 

PROVIDER: S-EPMC12731029 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature