Screening and Functional Analysis of tsRNAs Associated with Diabetic Foot Ulcer Tissues.

ABSTRACT: Background: Diabetic foot ulcer (DFU) is a serious complication of diabetes mellitus, and its inability to heal is closely linked to vascular pathology. However, the specific molecular regulatory networks involved remain unclear. This study hypothesizes that tRNA-derived small RNAs (tsRNAs) may be associated with endothelial dysfunction in DFU. Methods: RNA sequencing (RNA-seq) was performed on DFU (n = 3) and healthy foot skin (n = 3) samples. Bioinformatics analysis identified differential expression of tsRNAs, with the ten most significantly differentially expressed tsRNAs validated by qRT-PCR. Target gene prediction and GO/KEGG enrichment analysis were then conducted on the four tsRNAs that demonstrated significant differential expression, as confirmed by qR

SUBMITTER: He X 

PROVIDER: S-EPMC12731103 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature