Genome-Wide Mapping Reveals an Extensive AtfA Regulatory Influence on Development, Metabolism, and Stress Preparedness in <i>Aspergillus nidulans</i>.

ABSTRACT: Asexual sporulation (conidiogenesis) in filamentous fungi is a complex developmental process that requires precise coordination with primary metabolism and environmental stress responses. In the model fungus Aspergillus nidulans, we demonstrate that the bZIP-type transcription factor AtfA plays a central role in integrating conidiogenesis with the underlying metabolic and regulatory networks. Using combined ChIP-seq and RNA-seq analyses in wild-type, ∆atfA, and atfA-complemented strains under stress-free and oxidative stress (menadione) conditions, we identify a conserved AtfA binding motif and map its functional targets genome-wide. Our data reveal that AtfA binding to its target promoters is largely stress-independent, suggesting a preemptive regulatory mechanism in

SUBMITTER: Miskei M 

PROVIDER: S-EPMC12731236 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature