Microfluidic-Based Scratch Assays for Wound Healing Studies: A Systematic Review.

ABSTRACT: Cell migration plays a central role in physiological processes such as wound healing, tissue regeneration, and immune responses, as well as in pathological conditions like chronic inflammation and tumor metastasis. Among the in vitro approaches to study this phenomenon, the conventional wound healing assay (scratch assay) has been widely used due to its simplicity and low cost. However, its limitations, including poor reproducibility, damage to the extracellular matrix (ECM), and lack of dynamic physiological conditions, have prompted the development of microfluidic alternatives. Scratch-on-a-chip platforms integrate engineering and microtechnology to provide standardized, non-destructive methods for wound generation, preserve ECM integrity, and allow precise control of the cellular microe

SUBMITTER: Oliveira FA 

PROVIDER: S-EPMC12731330 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature