Comparison of Different Methods of Molecular Detection of Erwinia amylovora in Plant Material.

ABSTRACT: Fire blight, caused by Erwinia amylovora, is one of the most damaging bacterial diseases affecting apple production and the safety of wild Malus sieversii populations in Central Asia. Effective monitoring relies on accurate molecular diagnostics; however, comparative data on commonly used detection methods remain limited for the region. In this study, we evaluated the performance of three molecular assays-LAMP, real-time PCR, and targeted nanopore sequencing of a 16S rRNA gene fragment-using 124 plant samples exhibiting fire blight symptoms collected from 30 sites across Southern Kazakhstan and Kyrgyzstan. The results of LAMP, real-time PCR, and the amplification of 16S sequences were highly consistent with each other. Targeted 16S nanopore sequencing reliably identified E

SUBMITTER: Pozharskiy A 

PROVIDER: S-EPMC12731753 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature