Epigenome-Wide Search for Distinctive Methylation Biomarkers of Endothelial and Leukocyte DNA.

ABSTRACT: The endothelium, as the inner layer of the vascular wall, is in constant contact with blood components, so that leukocytes have the ability to adhere to endotheliocytes and penetrate to the subendothelial space. When studying heterogenic vascular samples containing endothelial cells or pathological processes related to inflammation within the endothelium, it may be necessary to distinguish DNA by endothelial and leukocyte origin, which is possible due to its specific epigenetic modifications. To identify CpG loci that could serve as markers for endothelial cells, we searched for their distinctive stable methylated or demethylated states by applying marginal filtering (selecting CpG loci with methylation Beta values closer to 0 and 1) to the microarray data and identified 47 CpG loci with r

SUBMITTER: Korolenya VA 

PROVIDER: S-EPMC12731890 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature