Development and Validation of a Selective Method to Quantify Low-Molecular-Mass Flavan-3-ols in Grapes and Wines.

ABSTRACT: Quantifying low-molecular-mass (LMM) flavanols in wines is relevant because these compounds, though typically minor, reflect flavanol structural composition (seed vs. skin contributions) and relate to cultivar and winemaking technique. Their determination is challenging because oligomeric and polymeric tannins interfere with standard spectrophotometric assays. This study introduces a coupled procedure that isolates and selectively quantifies LMM flavan-3-ols by combining the well-established methylcellulose precipitation assay (MCP) to remove oligomers and polymers with dimethylaminocinnamaldehyde (DMAC) determination of the MCP supernatant. The sequential workflow uses DMAC specificity and sensitivity and minimizes interference caused by higher-mass flavanols. Additionally, samples are qu

SUBMITTER: Favre G 

PROVIDER: S-EPMC12731937 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature