Functionally Distinct Shed Subpopulations Detected After Magnetic-Activated Cell Sorting of CD71 and CD146.

ABSTRACT: Mesenchymal stem cells derived from human exfoliated deciduous teeth (SHED) are a promising source for regenerative therapies due to their multipotency, proliferative capacity, and immunomodulatory properties. The present study aimed to isolate and characterize SHED subpopulations based on CD71 and CD146 expression and evaluate their multilineage differentiation potential. SHED were obtained from pediatric donors and separated into CD71⁺, CD71^-, CD146⁺, and CD146^- fractions using magnetic-activated cell sorting (MACS). CD71⁺/CD71^- and CD146⁺/CD146^- populations were isolated independently; no simultaneous double sorting for both markers was performed. Immunocytochemistry was employed to confirm the expre

SUBMITTER: Miteva M 

PROVIDER: S-EPMC12731958 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature