Aging Effects on Metabolic Sensor and Glycogen Metabolism in Old Male versus Female Rat Primary Hypothalamic Astrocyte Cultures.

ABSTRACT:

Background/objectives

Compartmentalized glucose metabolism in the brain contributes to neuro-metabolic stability and shapes hypothalamic control of glucose homeostasis. Glucose transporter-2 (GLUT2) is a plasma membrane glucose sensor that exerts sex-specific control of hypothalamic astrocyte glucose and glycogen metabolism. Aging causes counterregulatory dysfunction.

Methods

Current research used Western blot and HPLC-electrospray ionization-mass spectrometry to investigate whether aging affects GLUT2-dependent hypothalamic astrocyte metabolic sensor and glycogen enzyme protein expression and glycogen mass according to sex.

Results

Data document GLUT2-dependent up-regulated glucokinase (GCK) protein in glucose-deprived old male and female astrocyte cultures, unlike G

SUBMITTER: Shrestha R 

PROVIDER: S-EPMC12732729 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature