FOXM1 Maintains Homeostasis and Self-Renewal in Wharton's Jelly Mesenchymal Stem Cells.

ABSTRACT:

Background

The transcription factor FOXM1 is a master regulator of the cell cycle and is implicated in various cell fate decisions. However, its functional role and regulatory network in human Wharton's jelly mesenchymal stem cells (WJ-MSCs) remain poorly defined. This study aimed to elucidate the comprehensive function of FOXM1 in maintaining WJ-MSC stemness, proliferation, and survival, and to delineate the underlying molecular mechanisms.

Methods

We used RNA Interference to knock down FOXM1 in WJ-MSCs. The phenotypic impacts were assessed through CCK-8, colony formation, migration, and flow cytometry assays. We analyzed transcriptomic changes using RNA-seq and verified the results through qRT-PCR and Western blotting.

Results

Knockdown of FOXM1 significantly reduce

SUBMITTER: Li N 

PROVIDER: S-EPMC12732837 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature