A Dual-Gene Colorimetric LAMP Assay for Genus-Level Detection of <i>Salmonella</i> and Specific Identification of the Non-Motile Serovar S. Gallinarum Gallinarum.

ABSTRACT: Salmonella enterica serovar Gallinarum is a non-motile serovar and is the causative agent of fowl typhoid, and poses a major challenge to poultry production, particularly where rapid diagnostics are lacking. Existing methods are either time-consuming or fail to distinguish motile from non-motile serotypes. We developed a dual-target colorimetric LAMP that detects Salmonella spp. via invA and discriminates S. Gallinarum via TRX (a taxon-restricted sequence), using two separate singleplex reactions. Specificity testing confirmed 100% accuracy, with exclusive amplification of S. Gallinarum through TRX. Analytical sensitivity was comparable to real-time PCR, detecting down to 2.41 CFU/µL (invA) and 1.65 CFU/µL (TRX). Applied to cloacal swabs fro

SUBMITTER: Skenndri S 

PROVIDER: S-EPMC12732879 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature