The Disruption of <i>Cyp7b1</i> Controls IGFBP2 and Prediabetes Exerted Through Different Hydroxycholesterol Metabolites.

ABSTRACT: Cytochrome P450, family 7, subfamily b, polypeptide 1 (CYP7B1) is a widely expressed enzyme involved in the hydroxylation of sterols. Generated by transposon technology in zygotes, male rats lacking Cyp7b1 expression in homozygosis showed an absence of Cyp7b1 mRNA expression in the liver, small intestine, adipose tissue, and muscle. Elevated levels of 25-hydroxycholesterol were found in the liver of mutant rats. After overnight fasting, plasma triglyceride (TG) levels were increased in the homozygous rats. In agreement with this, increased hepatic secretion of very-low-density lipoprotein-TG (VLDL) in fasting rats treated with tyloxapol and decreased low-density receptor protein (LDLr) on the hepatocyte plasma membranes were observed. The decrease in LDLr was not due to decre

SUBMITTER: Martinez-Beamonte R 

PROVIDER: S-EPMC12732954 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature