Photodynamic Activation of Mammalian and Avian Cholecystokinin Type 1 Receptor Outside of the Pancreatic Acinar Cell Microenvironment.

ABSTRACT: Cholecystokinin 1 receptor (CCK1R) is activated by singlet oxygen (¹O₂) in type II photodynamic action in isolated rat, mouse, and Peking duck pancreatic acini. To examine whether this is maintained outside the microenvironment of pancreatic acinar cell, photodynamic activation of CCK1R from human, rat, mouse, and Peking duck expressed in CHO-K1 cells was examined, as monitored with Fura-2 fluorescence calcium imaging. Photodynamic action with sulphonated aluminum phthalocyanine was found to trigger persistent calcium oscillations in CCK1R-CHO-K1 cells transfected with human, rat, mouse or Peking duck CCK1R gene, which were blocked by ¹O₂ quencher Trolox C. After tagging protein photosensitizer miniSOG to C-terminus of these CCK1R, photody

SUBMITTER: Wang J 

PROVIDER: S-EPMC12733227 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature