Usefulness of DNA Obtained from FFPE Tissue Sections Stained with Masson's Trichrome in Forensic Identification: A Pilot Study.

ABSTRACT:

Background/objectives

Formalin-fixed paraffin-embedded (FFPE) tissues are sometimes the only DNA source for forensic applications. The quantity and integrity of the DNA extracted from these samples depend on multiple factors. In this work, we analyzed, for the first time, whether Masson's trichrome (MT) staining alters the results of genetic profiles obtained from DNA extracted from FFPE tissue sections.

Methods

Three pairs of sections from the year 2024 and three pairs from the year 2001 were analyzed. Each pair consisted of serial sections, one stained with hematoxylin and eosin and the other with MT. DNA was extracted using the PrepFiler Express BTA™ Forensic DNA Extraction Kit and quantified by real-time PCR using the Quantifiler™ HP DNA Quantification Kit. DNA samples w

SUBMITTER: Chavez-Briones MD 

PROVIDER: S-EPMC12733228 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature