<i>Thyrotropin-Releasing Hormone</i> Gene Methylation as a Potential Biomarker for Anal Intraepithelial Neoplasia.

ABSTRACT: Anal cancer is high in men who have sex with men living with human immunodeficiency virus (MSM-LWHIV). This cancer is strongly associated with high-risk human papillomavirus (HR-HPV) infection. Anal cancer screening using cytology and high-resolution anoscopy (HRA) for diagnosis of anal intraepithelial neoplasia requires specialized expertise. Biomarkers for the diagnosis of abnormal anal cells are of interest. Thyrotropin-releasing hormone (TRH) methylation at cg01009664 was detected using a pyrosequencing assay to compare methylation patterns among different anal lesions. Our results demonstrated that TRH methylation was significantly hypermethylated in anal intraepithelial neoplasia (AIN3) (>20%) and AIN1-2 (>10%) but less methylated in normal (<10%) (p < 0.001).

SUBMITTER: Pholpong C 

PROVIDER: S-EPMC12733343 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature