Unravelling the Potential of Fungal Division of Labour in the Laccase Producer <i>Coriolopsis trogii</i> MUT3379 Through Protoplast Formation and Regeneration.

ABSTRACT: The white-rot fungus Coriolopsis trogii MUT3379 produces Lac3379-1 laccase at high yields due to the previous development of a robust fermentation process. Throughout the extended use of this strain, we observed the occurrence of substrate-specific guaiacol and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) oxidizing enzymes other than Lac3379-1 Since we did not succeed in producing these enzymes in significant amounts by conventional strain selection and fermentation tools, we developed an approach based on protoplast preparation and regeneration to isolate stable producers of these alternative oxidative enzymes from the complex multinucleate mycelium of C. trogii MUT3379. A cost-effective and efficient protocol for protoplast preparation was developed by usi

SUBMITTER: Mellere L 

PROVIDER: S-EPMC12733382 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature