<i>BcHK71</i> and <i>BcHK67</i>, Two-Component Histidine Kinases, Regulate Conidial Morphogenesis, Glycerol Synthesis, and Virulence in <i>Botrytis cinerea</i>.

ABSTRACT: Fungal two-component signaling systems comprise histidine kinases (HKs), phosphotransfer intermediates, and response regulators. HKs are classified into eleven groups based on domain architecture; however, Group XI members in Botrytis cinerea remain uncharacterized. In this study, we investigated the functions of two Group XI histidine kinase genes, BcHK71 and BcHK67, in B. cinerea via gene replacement. Phenotypic analysis revealed that BcHK71 and BcHK67 regulate conidiation, infection structures formation, and glycerol synthesis. Notably, BcHK71 maintained cell wall integrity. Both genes also modulated expression of high osmolarity glycerol mitogen-activated protein kinase (HOG-MARK) signaling pathway components (BcYpd1, BcSkn7

SUBMITTER: Wang M 

PROVIDER: S-EPMC12733437 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature