Coordinated Auxin-Cytokinin-Nitrogen Signaling Orchestrates Root Suckering in Populus.

ABSTRACT: Root suckering is a key mode of clonal propagation in white poplar group, such as aspens (Populus section Leuce), enabling rapid vegetative spread, yet the molecular triggers remain elusive. Here, we developed a rapid protocol that produces abundant root suckers with the root cutting of white poplar (Populus davidiana × P. bolleana) roots in greenhouse. Anatomical analyses and daily resolution transcriptomes resolved three sequential developmental stages: primordium initiation (Days 0-1), SAM (shoot apical meristem) establishment (Days 1-4), and organ differentiation/growth (Days 4-6). Weighted gene co-expression network analysis revealed that auxin- and cytokinin-mediated signaling, integrated with nitrogen metabolism, orchestrates SAM formation and maintenance

SUBMITTER: Pang H 

PROVIDER: S-EPMC12733473 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature