Survey and Molecular Diagnostics of Target Site Mutations Conferring Resistance to Insecticides in Populations of <i>Aphis spiraecola</i> from Greece.

ABSTRACT: The spirea aphid, Aphis spiraecola (Hemiptera: Aphididae), is a major pest of citrus and other crops, causing feeding damage and transmitting plant viruses. Control relies mainly on insecticides, increasing the risk of resistance. We surveyed Greek populations of A. spiraecola (2022-2023) and developed molecular diagnostics to detect target site mutations linked to resistance against neonicotinoids, carbamates, organophosphates, pyrethroids, and spirotetramat. Seventy-two aphids were analyzed. All individuals were homozygous and susceptible at R81T, A302S, L1014F, M918 variants, and A2226V loci. However, the S431F mutation in the AChE2 gene, associated with resistance to dimethyl-carbamates, such as the selective aphicide pirimicarb, was present in all regions. Heteroz

SUBMITTER: Ilias A 

PROVIDER: S-EPMC12733833 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature