Identification, Subcellular Localization, and Infection-Related Expression of a Novel Haloacid Dehalogenase Gene (<i>VmHAD</i>) from <i>Valsa mali</i> Vm1.

ABSTRACT: The haloacid dehalogenase (HAD) superfamily represents a large group of enzymes across diverse taxa. However, the characteristics and functional roles of HAD members in the destructive apple canker pathogen, Valsa mali strain Vm1 (Vm1), remain poorly understood, particularly regarding their expression during infection. In this study, the full-length cDNA sequence of the VmHAD gene from Vm1 was cloned using rapid amplification of cDNA ends (RACE) technology, and its bioinformatic properties, subcellular localization, and expression patterns during infection were characterized. The VmHAD cDNA was 1044 bp in length, containing a complete open reading frame (ORF) of 798 bp that encodes a 265 amino acid protein with a conserved HAD-like domain. Phylogenetic analysis reveale

SUBMITTER: Zhang S 

PROVIDER: S-EPMC12733955 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature