Characterization of Cry4Aa Toxin from <i>Bacillus thuringiensis</i> JW-1 and Its Insecticidal Activity Against <i>Bradysia difformis</i>.

ABSTRACT: Bradysia difformis is a notorious pest in mushroom production in China. Biological control using Bacillus thuringiensis (Bt) offers an environmentally friendly and effective strategy against this pest. Here, we show that the complete genome of strain JW-1 consists of one circular chromosome and seven circular plasmids. JW-1-Plasmid 4 comprises 127,921 bp with a GC content of 33.9%, and is predicted to contain 131 genes, including six insecticidal genes: cry4Aa, cry4Ba, cry10Ab, cry11Aa, cyt1Aa, and cyt2Ba. A 3542-bp fragment containing the cry4Aa gene was amplified from this strain. Phylogenetic analysis based on Cry4 toxin sequences showed that JW-1 Cry4 toxin belongs to the Cry4Aa toxin cluster. A Cry4Aa fusion protein was su

SUBMITTER: Xu P 

PROVIDER: S-EPMC12734002 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature