Identification of miRNAs in Response to Cold Stress in 'Chaling' Common Wild Rice (<i>Oryza rufipogon</i> Griff.).

ABSTRACT: (1) Background: 'Chaling' common wild rice (CLWR), one of the two wild rice populations with the northernmost natural distribution worldwide, exhibits excellent cold tolerance. The role of microRNA (miRNA) in regulating cold tolerance in plants has been reported in some species. However, the miRNA landscape in CLWR remains unexplored. (2) Methods: We assessed cold tolerance in CLWR and the conventional rice variety 9311 at 4 °C, and conducted small RNA sequencing and analysis on eight samples from both CLWR and 9311, before and after cold treatment. (3) Results: All seedlings of CLWR survived after cold treatment and recovery, while all seedlings of 9311 died. After quality control and classification analysis of the small RNA sequences, numerous known and novel microRNAs (miRNAs) were iden

SUBMITTER: Gao F 

PROVIDER: S-EPMC12734129 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature