Development of a High-Throughput Screening Platform and a Pathogenesis Model for <i>Leishmania</i> Infection Based on Mouse Hepatic Organoids.

ABSTRACT: The development of new alternative models is essential to overcome the limitations of traditional two-dimensional (2D) cell cultures and animal models. Three-dimensional (3D) models, such as organoids, better mimic the structural and functional complexity of mammalian organs, thereby reducing the ethical and economic issues related to animal experimentation. These systems provide more physiologically relevant environments, improving the accuracy of disease modeling and drug response prediction. In this context, we have developed mouse hepatic organoids from livers of adult BALB/c mice and characterized them by microscopy and transcriptional analysis. This model was applied to a robust and reproducible high-throughput screening (HTS) platform for testing cytotoxicity at the preclinical stag

SUBMITTER: Gonzalez-Montero MC 

PROVIDER: S-EPMC12734288 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature