Study on Stability of Remifentanil, Sufentanil, and Their Metabolites in Human Whole Blood and Urine.

ABSTRACT:

Background

The accurate detection of remifentanil and sufentanil in biological samples is challenged by their rapid metabolism and instability, complicating clinical and forensic toxicology analysis. This study aimed to evaluate the stability of remifentanil, sufentanil, and their primary metabolites-remifentanil acid and norsufentanil-in human whole blood and urine.

Methods

A high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed and validated for simultaneous quantification, demonstrating satisfactory linearity (0.10-200 ng/mL, r² > 0.99), detection limits (0.01-0.20 ng/mL), and recovery rates (85.06-119.42%). Stability was assessed under varying temperatures (4 °C, -20 °C, -80 °C) and anticoagulant conditions (EDTA-K

SUBMITTER: Wang Z 

PROVIDER: S-EPMC12734456 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature