Construction of Recombinant <i>Escherichia coli</i> Expressing Ammonia Assimilation Genes and Evaluation of Its Effect on Removing Ammonium Nitrogen (NH₄⁺-N).

ABSTRACT: Treating wastewater with high ammonium nitrogen (NH₄⁺-N) is a public and environmental priority. Unlike nitrification-denitrification, ammonia assimilation channels NH₄⁺-N into the glutamate biosynthetic pathway, avoiding gaseous nitrogen species (NOx, N₂). Here we engineered Escherichia coli to enhance ammonia assimilation by co-expressing three key genes, gdhA, glnA, and guaA. The genes were synthesized, cloned into expression plasmids, and introduced into E. coli BL21 for IPTG-inducible expression. Expression of the target proteins at expected sizes was observed, and NH₄⁺-N removal was assessed in flask fermentations. Recombinant strains exhibited significantly higher NH₄

SUBMITTER: Pan P 

PROVIDER: S-EPMC12735046 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature