Anti-Cancer Activity of <i>Sphaerococcus coronopifolius</i> Algal Extract: Hopes and Fears of a Possible Alternative Treatment for Canine Mast Cell Tumor.

ABSTRACT: Within the "One Health, One Medicine" and comparative oncology paradigms, algal extracts have attracted attention, containing natural compounds (NCs) with biological activities, including anti-cancer properties. To characterize the biological effects of a Sphaerococcus coronopifolius extract (SCE), two canine mastocytoma and two normal cell lines were used. After a preliminary screening of three algal extracts, SCE cytotoxicity was measured using Alamar Blue, Sulforhodamine B, and Neutral Red Uptake assays. After assessing the selectivity versus tumor cells and its chemical characterization, SCE mechanisms of action were investigated using RNA-seq, quantitative PCR, flow cytometry and immunoblotting approaches. SCE showed an IC₅₀ comprised between 25 and 35 μg/mL in tumor

SUBMITTER: Mucignat G 

PROVIDER: S-EPMC12735324 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature