A Rapid RT-RAA Assay for Visual Detection of Ebola Virus: Advancing Early Diagnosis in Resource-Limited Settings.

ABSTRACT: Ebola virus (EBOV) infection constitutes a significant global public health threat, and no curative treatment is currently available for it. Rapid and accurate detection of EBOV nucleic acid is crucial for controlling the spread of Ebola virus disease (EVD). The gold standard for EBOV diagnosis is real-time reverse transcription polymerase chain reaction (RT-qPCR), which requires costly equipment and skilled personnel, potentially hindering its application for rapid detection, especially in resource-limited settings. Consequently, there is an urgent need to develop a simple, accurate, and rapid diagnostic method for EVD. In this study, a real-time reverse transcription recombinase-aided amplification (RT-RAA) assay was developed for the specific visual detection of the conserved region of

SUBMITTER: Li Z 

PROVIDER: S-EPMC12735406 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature