Decay of Food DNA in the Gastrointestinal Tract: Implications for Molecular Dietary Records.

ABSTRACT: Background/Objectives: The widely recognized potential for biased responses to food frequency questionnaires and non-compliant self-reporting is motivating the search for alternative food intake records. The analysis of fecal DNA has been investigated as a potentially less biased and technically manageable method to replace or complement oral or written dietary surveys. The accuracy of fecal-DNA-based recalls critically depends on the persistence of ingested DNA of dietary origin during digestion. Methods: To inform the implementation of alternative molecular dietary inventories, we quantified the concentration of dietary DNA in the small intestine and in the feces of dogs, and in several sections of the mouse gastro-intestinal tract. Results: Using PCR assays specific

SUBMITTER: Patel MJ 

PROVIDER: S-EPMC12735545 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature